趋化因子受体CCR7在T细胞淋巴瘤播散中的作用

杨晶; 王晟怡; 赵国范; 孙保存

doi:10.3969/j.issn.1000-8179.2011.07.002

摘要: 目的：探讨趋化因子受体CCR7在T细胞淋巴瘤播散中的作用；旨在为T细胞淋巴瘤靶点治疗的新方向提供一定的实验基础及理论依据。方法：皮肤T细胞淋巴瘤Hut78和成人T淋巴细胞白血病/淋巴瘤Jurkat细胞株培养，通过Transwell小室检测了细胞体外侵袭能力并进行比较；通过RT-PCR和Western-blot技术对两种细胞株的CCR7和PI3K/Akt信号转导通路的表达进行了检测。结果： 1） Hut78细胞体外侵袭能力较强；两种T细胞淋巴瘤细胞经过CCL21共培养后，侵袭能力均有所增强，且均呈现与CCL21浓度的正相关趋势； 2） Hut78细胞中CCR7mRNA， PI3KmRNA、 AktmRNA均高于Jurkat细胞，差异均有显著性（P<0.001）；CCR7、 pAkt蛋白在Hut78细胞中的表达也明显高于Jurkat细胞（P<0.05）。结论： T细胞淋巴瘤中高表达的CCR7与肿瘤的侵袭性有关，可能通过PI K/Akt信号通路促进肿瘤的侵袭和播散。

Abstract: The Role of Chemokine Receptor CCR7 in Dissemination of T Cell LymphomaJing YANG1,4, ShengyiWANG2, Guofan ZHAO1,4, Baocun SUN1,3, 4Correspondence to: Baocun SUN, E-mail: sunbaocun@yahoo.com.cn1Teaching and Research Section of Pathology, Tianjin Medical University, Tianjin 300070, China2Department of Medical Psychology, Tianjin Medical University, Tianjin 300070, China3Department of Pathology, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, China4Department of Pathology, General Hospital of Tianjin Medical University, Tianjin 300052, ChinaThis work was supported by Tianjin Municipal Projects for Scientific Development Support (Sino-Swiss International Cooperation, No.09ZCZDSF04400)Abstract Objective: To explore the role and mechanisms of chemokine receptors in the T-cell lymphoma invasion process in or-der to provide experimental and theoretical basis for target therapy of T-cell lymphoma. Methods: The cutaneous T-cell lymphomaHut78 and adult T-cell leukemia/lymphoma Jurkat cell lines were co-cultured. The in vitro invasive ability of the two cell lines was mea-sured by Transwell invasion assay. The transcription and expression of CCR7 and key enzymes in PI3K/Akt pathway were evaluated us-ing RT-PCR and Western blot. Results: Hut78 cell lines showed a stronger invasive ability in the Transwell assay. After the two celllines were co-cultured with chemokine CCL21, their invasive ability was increased. The invasive ability displayed a positive correlationwith the CCL21 concentration. The CCR7 mRNA, PI3K mRNA and Akt mRNA expression was all higher in Hut78 cell line than in Jur-kat cell line, with significant differences ( P < 0.001 ). The expression of CCR7 and pAkt protein was significantly higher in Hut78 cellline than in Jurkat cell line ( P < 0.05 ). Conclusion: The high expression of CCR7 in T-cell lymphoma is related to the invasion of tu-mors. CCR7 may promote tumor invasion and metastasis through the PI3K/Akt signal pathways.Keywords CCR7; PI3K/Akt signal transduction pathway; T cell lymphoma